Biochemical characterization of the rat liver receptor for 2,3,7,8-TCDD - a comparison to the rat liver glucocorticoid receptor

An assessment of the physicochemical characteristics of the rat hepatic receptors for 2,3,7,8-TCDD and for corticosteroid hormones reveals striking analogies between both receptor systems.     

RED RIVER OF THE NORTH BASIN,MINNESOTA, NORTH DAKOTA,AND SOUTH DAKOTA1

ABSTRACT: The environmental setting of the Red River of the North basin within the United States is diverse in ways that could significantly control the areal distribution and flow of water and, therefore, the distribution and concentration of constituents that affect water quality. Continental glaciers shaped a landscape of very flat lake plains near the center of the basin, and gently rolling uplands, lakes, and wetlands along the basin margins. The fertile, black, fine-grained soils and landscape are conducive to agriculture. Productive cropland covers 66 percent of the land area. The principal crops are wheat, barley, soybeans, sunflowers, corn, and hay. Pasture, forests, open water, and wetlands comprise most of the remaining land area. About one-third of the 1990 population (511,000) lives in the cities of Fargo and Grand Forks, North Dakota and Moorhead, Minnesota. The climate of the Red River of the North basin is continental and ranges from dry subhumid in the western part of the basin to subhumid in the eastern part. From its origin, the Red River of the North meanders northward for 394 miles to the Canadian border, a path that is nearly double the straight-line distance. The Red River of the North normally receives over 75 percent of its annual flow from the eastern tributaries as a result of regional patterns of precipitation, evapotranspiration, soils, and topography. Most runoff occurs in spring and early summer as a result of rains falling on melting snow or heavy rains falling on saturated soils. Lakes, prairie potholes, and wetlands are abundant in most physiographic areas outside of the Red River Valley Lake Plain. Dams, drainage ditches, and wetlands alter the residence time of water, thereby affecting the amount of sediment, biota, and dissolved constituents carried by the water. Ground water available to wells, streams, and springs primarily comes from sand and gravel aquifers near land surface or buried within 100 to 300 feet of glacial drift that mantles the entire Red River of the North basin. Water moves through the system of bedrock and glacial-drift aquifers in a regional flow system generally toward the Red River of the North and in complex local flow systems controlled by local topography. Many of the bedrock and glacial-drift aquifers are hydraulically connected to streams in the region. The total water use in 1990, about 196 million gallons per day, was mostly for public supply and irrigation. Slightly more than one half of the water used comes from ground-water sources compared to surface-water sources. Most municipalities obtain their water from ground-water sources. However, the largest cities (Fargo, Grand Forks and Moorhead) obtain most of their water from the Red River of the North. The types and relative amounts of various habitats change among the five primary ecological regions within the Red River of the North basin. Headwater tributaries are more diverse and tend to be similar to middle-reach tributaries in character rather than the lower reaches of these tributaries for the Red River of the North. Concentrations of dissolved chemical constituents in surface waters are normally low during spring runoff and after thunderstorms. The Red River of the North generally has a dissolved-solids concentration less than 600 milligrams per liter with mean values ranging from 347 milligrams per liter near the headwaters to 406 milligrams per liter at the Canadian border near Emerson, Manitoba. Calcium and magnesium are the principal cations and bicarbonate is the principal anion along most of the reach of the Red River of the North. Dissolved-solids concentrations generally are lower in the eastern tributaries than in the tributaries draining the western part of the basin. At times of low flow, when water in streams is largely from ground-water seepage, the water quality more reflects the chemistry of the glacial-drift aquifer system. Ground water in the surficial aquifers commonly is a calcium bicarbonate type with dissolved-solids concentration generally between 300 and 700 milligrams per liter. As the ground water moves down gradient, dissolved-solids concentration increases, and magnesium and sulfate are predominant ions. Water in sedimentary bedrock aquifers is predominantly sodium and chloride and is characterized by dissolved-solids concentrations in excess of 1,000 milligrams per liter. Sediment erosion by wind and water can be increased by cultivation practices and by livestock that trample streambanks. Nitrate-nitrogen concentrations also can increase locally in surficial aquifers beneath cropland that is fertilized, particularly where irrigated. Nitrogen and phosphorous in surface runoff from cropland fertilizers and nitrogen from manure can contribute nutrients to lakes, reservoirs, and streams. Some of the more persistent pesticides, such as atrazine, have been detected in the Red River of the North. Few data are available to conclusively define the presence or absence of pesticides and their break-down products in Red River of the North basin aquifers or streams. Urban runoff and treated effluent from municipalities are discharged into streams. These point discharges contain some quantity of organic compounds from storm runoff, turf-applied pesticides, and trace metals. The largest releases of treated-municipal wastes are from the population centers along the Red River of the North and its larger tributaries. Sugar-beet refining, potato processing, poultry and meat packing, and milk, cheese, and cream processing are among the major food processes from which treated wastes are released to streams, mostly in or near the Red River of the North.     

Phthalates in house dust

The main objective of this study was to generate a fast analytical method to determine the five phthalates benzylbutylphthalate (BBP), dibutylphthalate (DBP), di-(2-ethylhexyl)-phthalate (DEHP), di-isodecylphthalate (DIDP), and di-isononylphthalate (DINP) in house dust. To achieve this liquid chromatography electrospray tandem mass spectrometry (LC–ESI–MS/MS) was used for measurement. The risk of lab- and cross-contamination was nearly eliminated completely as a very short and fast sample preparation including a sieving step and an ultrasonic extraction for the analytes from the dust samples was used. Quantification through internal standard calibration resulted in low limits of determination (DEHP 4 mg kg^? 1 to DBP 14 mg kg^? 1). A potential interaction between the analytes DIDP and DINP during chromatographic measurement could be excluded while performing a two level factorial design. Furthermore it was examined to what extend carpet and plastic materials respectively have influence on the total amount of phthalates in dust. It could be shown that apartments in which a minimum of both of these sources appeared revealed the lowest total amount of sum of phthalates in dust (median 362 mg kg^? 1).     

Re-Meandering German Lowland Streams: Qualitative and Quantitative Effects of Restoration Measures on Hydromorphology and Macroinvertebrates

We investigated the effects of two river restoration projects on hydromorphology and macroinvertebrate fauna in two German lowland rivers, the Schwalm and the Gartroper Mühlenbach. The stream channels were re-meandered and the floodplain levels were lowered to better connect the streams to their floodplains. The restoration was performed 10 years ago in the Schwalm and 2 years ago in the Gartroper Mühlenbach. We compared the restored reaches to nearby anthropogenically straightened reaches. Twenty-five hydromorphological parameters were recorded on twenty transects; between nine and 23 substrate-specific macroinvertebrate samples were taken per reach. Several hydromorphological parameters, such as the number and width of channel features and the number of substrate types, were significantly higher in the restored reaches compared to nearby anthropogenically straightened reaches. Total numbers of invertebrate families, genera, and taxa were also higher in the restored reaches than in the anthropogenically straightened reaches. Biotic substrates like dead wood or macrophytes were more abundant in the restored reaches, and these substrates hosted 28 taxa not found in the straightened reaches. While diversity was high in both restored reaches, overall abundance increased only in the river that was restored 10 years ago. Using NMS-analysis, substrate-specific faunistic samples of the restored reaches were compared to those of the straightened reaches. Our results revealed different invertebrate communities on the same substrates in the recently restored river. In the 10-year-old restoration, however, the same substrates were similarly inhabited. This comparable colonization of substrates may reflect succession in the macroinvertebrate community. The results are discussed according to the re-colonization potentials of the upstream and downstream reaches and the dispersal capacity of taxa.     

Biogeochemical C and N cycles in urban soils

The percentage of urban population is projected to increase drastically. In 2030, 50.7 to 86.7% of the total population in Africa and Northern America may live in urban areas, respectively. The effects of the attendant increases in urban land uses on biogeochemical C and N cycles are, however, largely unknown. Biogeochemical cycles in urban ecosystems are altered directly and indirectly by human activities. Direct effects include changes in the biological, chemical and physical soil properties and processes in urban soils. Indirect effects of urban environments on biogeochemical cycles may be attributed to the introductions of exotic plant and animal species and atmospheric deposition of pollutants. Urbanization may also affect the regional and global atmospheric climate by the urban heat island and pollution island effect. On the other hand, urban soils have the potential to store large amounts of soil organic carbon (SOC) and, thus, contribute to mitigating increases in atmospheric CO(2) concentrations. However, the amount of SOC stored in urban soils is highly variable in space and time, and depends among others on soil parent material and land use. The SOC pool in 0.3-m depth may range between 16 and 232 Mg ha(-1), and between 15 and 285 Mg ha(-1) in 1-m depth. Thus, depending on the soil replaced or disturbed, urban soils may have higher or lower SOC pools, but very little is known. This review provides an overview of the biogeochemical cycling of C and N in urban soils, with a focus on the effects of urban land use and management on soil organic matter (SOM). In view of the increase in atmospheric CO(2) and reactive N concentrations as a result of urbanization, urban land use planning must also include strategies to sequester C in soil, and also enhance the N sink in urban soils and vegetation. This will strengthen soil ecological functions such as retention of nutrients, hazardous compounds and water, and also improve urban ecosystem services by promoting soil fertility.     

太湖流域北部潜在洪涝风险区分析及影响评估

太湖流域历来遭受严重的洪涝灾害,并且近年来有不断加重的趋势。本文选择太湖流域北部的无锡市作为典型研究地区,研究其洪涝的危险性并进行潜在损失评估。人类活动,包括修筑圩区、森林破坏、人口增长、城市化等,对这个地区的洪涝形势有较大的影响;自然条件的变化使河道水位升高,洪涝历时缩短,洪峰增高。本文选择不同回归年(2,5,10,20,50年),结合地理信息系统分析模型,计算了潜在洪涝危险区域和洪涝损失,主要结论为:(1)人类活动已经改变了自然的洪涝形势,增大了汇流和洪涝;(2)洪涝危险区与降雨中心有关;(3)筑圩成功地保护了圩内的土地,缩短了洪涝历时,增高了圩外河道的洪涝水位;(4)经济发展使洪涝损失增加。